Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 17;9:19303. doi: 10.1038/s41598-019-55844-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Conditional deletion of the Lamc1 gene in adult mice rapidly induces weight loss and morbidity/mortality that is due to gastrointestinal dysfunction. (A) Tamoxifen-treated control (black line) and tamoxifen-treated mutant (red line) mice were weighted the day of first tamoxifen injection, and throughout a three week period. Change in weight and survival are shown. Starting n = 10 for each cohort in weight graph, and mutant n = 17 and control = 10 for survival estimate. (B) Representative pictures of the intestines from tamoxifen treated control (n = 4) and mutant (n = 8) mice just after euthanasia. For (C,D), tamoxifen-treated (C_T), tamoxifen-treated mutant (M_T). (C) Weight of stool collected over a 10-minute observation period and compared between genotypes. Each data point represents the results from a single mouse. (D) Fecal and serum albumin concentrations compared between genotypes. For panels C&D, each point represents the data from an individual animal. (E) Small intestine sections from C_T and M_T mice were immunostained with antibodies that recognize thrombomodulin (green) and PGP9.5 (red). Nuclei are stained blue with DAPI. Magnification bars are 50 microns in low magnification images, and 10 microns in high magnification images. 20x and 40x lenses with 1.5x magnifier were used to collect these images. Images are representative of the results obtained from immunofluorescent analysis of 3 tamoxifen-treated control and 4 tamoxifen-treated mutant mice.