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. 2019 Dec 17;9:19303. doi: 10.1038/s41598-019-55844-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Laminin-ɣ1 deficiency causes intestinal epithelial hyperplasia. Hematoxylin and eosin stained paraffin-embedded tissue sections from tamoxifen-treated control and mutant duodenum. Magnification bars indicate 50 microns. Tamoxifen treated control and mutant paraffin-embedded duodenum sections were stained with antibodies against lactase or lysozyme (magenta). DAPI (blue) was used to label nuclei. Magnification bars indicate 50 microns for panels denoting lactase staining, and 20 microns for those showing lysozyme immunoreactivity. Lysozyme images are a maximum intensity projection image derived from a z-stacked image obtained with a 100x oil lens. Images are representative of the results obtained from immunofluorescent analysis of 3 tamoxifen-treated control and 4 tamoxifen-treated mutant mice.