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. 2019 Dec 17;9:19232. doi: 10.1038/s41598-019-55291-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Individual and combined effects of T and WSD on gene expression in omental WAT. (A) Principal component analysis (PCA) of gene expression including the 500 most variable genes among the 12 samples (n = 6 samples per each group) showing segregation of controls (C) and T + WSD. (B) Venn diagram showing the overlap between DEGs in the three independent comparisons: T (red), WSD (blue) and T + WSD (green) using a fold change cutoff of 1.5 and an FDR cutoff of 0.05. (C) Pathway analysis using IPA of DEGs in the T + WSD group. IPA assigns an activation score based on biological relevance and the number of genes in the canonical pathway. “Log Ratio” is calculated as Log₂ (fold change) in gene expression compared to control. Orange, activated pathways; blue, inhibited pathways; no color, no change in activation state.