Fig. 2. The YtfK/SpoT ratio determines growth on SMG plates.
WT strain was co-transformed with pEG25 harboring spoT gene under an IPTG-inducible promoter and with pBbS2K harboring ytfK gene under an anhydrotetracyclin (aTc) promoter. Cells were serially diluted and spotted both on SMG and NA medium with gradual concentration of IPTG (to induce spoT) and aTc (to induce ytfK). Additional controls with empty plasmids are provided in Supplementary Fig. 2. Experiments have been repeated four times with similar results.