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. 2019 Dec 17;10:5763. doi: 10.1038/s41467-019-13764-4

Fig. 4. YtfK contributes to antibiotic tolerance.

Fig. 4

a YtfK is required for cerulenin survival in absence of relA. Growing cells of MG1655 (WT) and isogenic deletion strains ΔrelA, ΔytfK, ΔrelA ytfK, ΔrelA spoT, and ΔrelA ytfK harboring the pEG220 and the ΔrelA ytfK harboring the pEG220-ytfK (with native promoter) were exposed to 500 µg/mL of cerulenin for 4 h (see the Methods section). Percentage of survival (log scale) after 4 h is shown. Error bars indicate the SDs of averages of three independent experiments. b Growth curve of WT and isogenic deletion strains ΔrelA, ΔytfK, ΔrelA ytfK and ΔrelA spoT, in presence (dashed line) or absence (solid line) of cerulenin. Overnight culture were 1000 times diluted in LB, and growth was monitored at 600 nm using a microplate reader. At the indicated time, 250 µg/ml of cerulenin was added. Error bars indicate the SDs of averages of three independent experiments. Source data are provided as a Source Data file.