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. 2019 Dec 17;10:5744. doi: 10.1038/s41467-019-13702-4

Fig. 3. ZapA stabilizes FtsZ filament bundles.

Fig. 3

a In the absence of ZapA (upper panel), the FtsZ filament pattern constantly reorganized and ring-like structures formed only transiently (red dashed squares). In contrast, in the presence of ZapA (lower panel) FtsZ assemblies were much more persistent. Scale bars, 2 μm. b The temporal autocorrelation function of the FtsZ filament network decayed quickly without ZapA (blue), but slowly in the presence of 6.0 μM (red). Each curve represents the mean ± std (error bands) of independent experiments (FtsZ, n = 7, ZapA, n = 9). c The characteristic correlation time, τ, as a function of ZapA concentration. The autocorrelation curves were fitted to a mono-exponential decay and τ was given by the half-time of each decay. Once again, ZapA induced a switch-like behavior showing a hill coefficient » 4 and a EC50 = 1.05 (Supplementary Table 2). Data shown corresponds do the average τ from individual experiments (mean ± std). d ZapA I83E and R46A (light red) did not change the persistence of FtsZ filament bundles (p > 0.05, Supplementary Table 1). Source data available at Figshare [10.6084/m9.figshare.10347062.v1].