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. 2019 Dec 17;10:5744. doi: 10.1038/s41467-019-13702-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Dual-color TIRF micrograph showing co-localization of Alexa488-FtsZ (cyan) and ZapA-Cy5 (magenta). b Raw image of FtsZ filament network overlaid with growing trajectories of FtsZ (cyan) and ZapA (magenta) from differential imaging (scale bar 5 μm). Kymographs are obtained along the orange arrow, which indicates treadmilling direction. Scale bars, t = 20 s, x = 3 μm. c Snapshots showing FRAP experiments of Cy5-labelled ZapA pattern. d Half-time recovery of Cy5-labelled ZapA showed a much faster recovery (ZapA = 3.01 ± 0.47, n = 6) when compared with FtsZ (Fig. 6b). e In contrast with FtsZ (Fig. 4d), the lifetime of ZapA was much shorter (ZapA = 0.95 ± 0.14, n = 5). Source data available at Figshare [10.6084/m9.figshare.10347062.v1].