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. 2019 Dec 17;9:19233. doi: 10.1038/s41598-019-55882-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dietary fatty acids in normolipidic diets affect growth, adiposity, liver and glucose metabolism. C57/BL6 mice were fed normolipidic diets containing different fatty acid compositions for 32 weeks (a). Body weight increase (b,c), growth indices (d,e) and food consumption (f) were determined weekly. After 32 weeks on the specified diets, mice were euthanized and various organs (g) and adipose tissues (h) were weighed The livers were severely affected on visual inspection and were further analyzed by histology after HE staining, including assessment of lipid droplet content and sizes (i,j,) N.D. = not-detected. The arrowhead points to an inflammatory focus. After 31 weeks on the specified diets, mice had their fasting glucose concentration measured (k) and sequentially submitted to the glucose tolerance test (GTT; l, mean glucose concentration; m, area under the curve) and the insulin tolerance test (ITT; n, mean glucose concentration; o, decay constant). n = 12 (a–h) or n = 6 (i–o); Statistically significant differences are indicated by different letters or asterisks (p < 0.05). ANOVA and Tukey’s test.