Table 1.
Binding kinetics and affinitya of Ig proteins for their antigensb.
| Ab | Type | kon (M−1 s−1) | koff (s−1) | KD (M) |
|---|---|---|---|---|
| 6C407 | Mouse IgG2a/κ | (5.67 ± 0.11) × 105 | (1.38 ± 0.04) × 10−3 | 2.45 × 10−9 |
| Chimeric MH (IgG1/κ) | (5.82 ± 0.07) × 105 | (1.31 ± 0.02) × 10−3 | 2.25 × 10−9 | |
| Chimeric MC (IgY/λ) | (5.11 ± 0.01) × 105 | (6.32 ± 0.03) × 10−4 | 1.24 × 10−9 | |
| 3D8 | Mouse IgG2a/κ | (6.04 ± 0.15) × 106 | (1.36 ± 0.06) × 10−1 | 2.25 × 10−8 |
| Chimeric MH (IgG1/κ) | (5.08 ± 0.07) × 105 | (2.42 ± 0.03) × 10−2 | 4.77 × 10−8 | |
| Chimeric MC (IgY/λ) | (2.72 ± 0.02) × 105 | (7.00 ± 0.08) × 10−3 | 2.56 × 10−8 |
aThe KD values were calculated by analyzing at least five data sets using different protein concentrations.
bThe 12-amino acid length peptide antigen labeled with biotin at the N-terminal for 6C407. The single-stranded oligodeoxynucleotide labeled with biotin at the 5′ end [bio-ss-(dN)40] for 3D8. Each value represents the mean ± SD of two independent experiments.