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. 2019 Dec 17;10:5762. doi: 10.1038/s41467-019-13775-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Illustration of the IDA principle. Competitive titrations of GC5A-6C•Fl (0.4/0.5 μM) with b PFOS (up to 2.4 μM) and c PFOA (up to 7.6 μM). (Inset) The associated titration curves of b PFOS and c PFOA fit according to a 1:1 competitive binding model. All experiments were performed in HEPES buffer at 25 °C, λ_ex = 500 nm, and λ_em = 513 nm. Data represent mean ± s.d. (n = 3 independent experiments).