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. 2019 Dec 17;9:19236. doi: 10.1038/s41598-019-55508-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TR3-derived vectors elicit HIVgag-specific T_EM in RM. (A) TR3 elicits T cell responses to HIVgag in RM. Two RM were inoculated with 5 × 10⁶ FFU of TR3ΔUL78gag at day 0. PBMC were collected at the indicated days and HCMV-lysate as well as HIVgag-specific CD4+ and CD8+ T cell responses were measured by intracellular cytokine staining for TNFα and/or IFNγ. The frequency of responding T cells is shown as percent of total memory T cells. (B) pp71-deleted TR3 elicits T cell responses to HIVgag. RM were inoculated with 10² FFU (n = 2), 10⁴ FFU (n = 2) or 10⁶ FFU (n = 4) of TR3ΔUL82gag at day 0 and HCMV lysate and HIVgag-specific T cell responses were determined as in A) at the indicated time points post-infection. (C) Frequency of memory populations within the HIVgag-specific CD4+ and CD8⁺ memory T cells in peripheral blood of the RM inoculated with 10⁴ FFU or 10⁶ FFU of TR3ΔUL82gag. Memory differentiation state was based on CD28 vs. CCR7 expression, delineating central memory (+/+T_CM), transitional effector memory (+/− T_TrEM), and effector memory (−/− T_EM), as designated. The same colors and symbols are used in B) and in C) for the same animals.