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. 2019 Dec 17;9:19297. doi: 10.1038/s41598-019-55253-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of sewage bacteria. Neighbor Joining tree. Each dot presents a sample. All the samples are connected to the tree by a single line. Abbreviations are the first four letters of reference samples for Acinetobacter indicus, Bacillus anthracis, Candidatus Accumulibacter phosphatis, Derxia gummosa, Flavobacterium pectinovorum, Mycobacterium sp. strain FED_F02A, Paludibacter propionicigenes, Pseudomonas punonensis, Shinella kummerowiae, and Zoogloea resiniphila, respectively (A). Scatter plot of scaled PC, sPC1 and sPC2 for samples (B). Scatter plot for sPC1 and sPC3 (C). A 3D version is available in Supplementary information, Fig. 2_BC.html. sPC3 for nucleotides (partial) (D). Positive and negative bases are coloured green and magenta, respectively; those had low contributions are coloured grey, and none contribution are omitted. Shinella, which appeared as a positive in the same sPC3 axis (C), tended to contain bases indicated in green, whereas Bacillus tended to contain bases indicated in magenta. Such tendencies are obvious in a summary of table output of sPC_n (E). Bases positive (>0.05) and negative (<−0.05) in sPC3 are shown with sequences of several samples that are indicated in panels B and C by green or magenta asterisks.