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. 2019 Dec 17;9:19297. doi: 10.1038/s41598-019-55253-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Direct PCA on MHC samples. Scatter plot of sPC1 and sPC2 for samples of Asiatic lions (A). PCA was performed directory to the Nucleotide sequence matrix. Scatter plot for samples of human data (B). sPC1 and sPC2 for nucleotide bases of lion data (C). PCs for bases of human data (D). Contributions (%) for sPC1 to sPC8 of PCA applied to sequence matrix directory (E) and distance matrix (F), respectively.