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. 2019 Dec 17;10:5759. doi: 10.1038/s41467-019-13652-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a MRK-740 but not MRK740-NC decreases PRDM9-dependent K4 trimethylation of exogenous histone H3. HEK293T cells were co-transfected with H3-GFP and PRDM9-FLAG and treated with compounds at indicated concentrations for 20 h. Mut denotes PRDM9 catalytic mutant (Y357S). First and last lanes are controls at 0 µM of compound. b The graph represents non-linear fit of H3K4me3 fluorescence intensities normalized to intensities of GFP (MRK-740 n = 10, 4 separate experiments, MRK-740-NC n = 4, 2 separate experiments). c MRK-740 and MRK-740-NC do not affect HEK293T cell growth up to 10 µM. Cells were treated with compounds for 1 day. Cell number was measured using IncuCyte™ ZOOM live cell imaging device. The results are mean ± sd, n = 3. Source data are provided as a Source Data file.