Table 1.
MS/MS parameters for the analysis of target compounds cortisol and cortisone and the internal standards d4-cortisol and d-8 cortisone.
| Compound | Rt | Ionization | Quantifier | Qualifier | ||||
|---|---|---|---|---|---|---|---|---|
| (min) | mode | Transition | Cone/Impactor* (V) | Collision (V) | Transition | Cone/impactor*(V) | Collision (V) | |
| Cortisol | 1.84 | ESI+ | 363.3 → 120.6 | 32 | 22 | 363.3 → 90.5 | 32 | 54 |
| d4-Cortisol | 1.85 | ESI+ | 367.3 → 120.7 | 26 | 20 | |||
| Cortisone | 1.75 | ESI+ | 361.2 → 162.8 | 26 | 24 | 361.2 → 104.6 | 26 | 40 |
| d8-Cortisone | 1.73 | ESI+ | 369.3 → 168.8 | 34 | 24 | |||
| Cortisol | 1.84 | USI+ | 363.2 → 120.7 | 34 | 26 | 363.2 → 327.2 | 34 | 16 |
| d4-Cortisol | 1.84 | USI+ | 367.2 → 120.7 | 38 | 24 | |||
| Cortisone | 1.74 | USI+ | 361.2 → 162.8 | 38 | 24 | 361.2 → 104.6 | 38 | 46 |
| d8-Cortisone | 1.73 | USI+ | 369.3 → 168.9 | 50 | 28 | |||
Rt: retention time; V: voltage; *for USI source the ionization involves a stainless steel rod. ESI, electrospray ionization; UNI, UniSpray ionization.