Table 1.
GSSPs validated in M. tuberculosis RUS_B0 strain.
| RUS_B0 locus | Locus in H37Rv | Six prot acc | Unique GSSP | Previous aminoacid | GSSP sequence | Next aminoacid | Best Mascot Ion score | Best Mascot Identity score | Best Mascot Delta Ion score | Best X! Tandem –log (e) score | Annotated in other M. tubeculosis strains | Found in transcriptome data |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Start site correction | ||||||||||||
| DPM14_06085 | Rv1330c | 1279837:1281369_reverse | 1 | R | TAVGPPPAAR | R | 50,2 | 25 | 36,7 | 1,19 | Yes | No |
| DPM14_06590 | Rv1425 | 1379719:1381659_forward | 1 | R | IAVSDPHQVLQAVIR | P | 0 | 0 | 0 | 1,6 | No | No |
| DPM14_07380 | Rv1567c | 1557336:1557938_reverse | 1 | R | SALDEPDER | T | 37,5 | 25 | 28,6 | 0 | Yes | Yes |
| DPM14_01015 | Rv0186 | 214254:216599_forward | 1 | R | EVEAQMTDDER | F | 22,8 | 25 | 22,8 | 1,41 | Yes | Yes |
| DPM14_15480 | Rv1093 | 3192084:3193553_reverse | 1 | R | TTAVMSAPLAEVDPDIAELLAK | E | 32,3 | 25 | 32,3 | 7,7 | Yes | Yes |
| DPM14_16025 | Rv0998 | 3298349:3299428_reverse | 1 | R | PDATTVRTTVSWAWR | R | 28,8 | 25 | 28,8 | 0 | Yes | No |
| DPM14_18255 | Rv3329 | 3713427:3714839_forward | 1 | R | SLPEPSAALANTTTR | N | 15,5 | 25 | 15,5 | 2,01 | Yes | Yes |
| DPM14_18270 | NA | 3716955:3718877_forward | 1 | R | AAVHHRVQLR | I | 17 | 25 | 14 | 0 | No | Yes |
| DPM14_19510 | Rv3556c | 4002140:4003402_reverse | 1 | R | GAVMTEAYVIDAVR | T | 0 | 0 | 0 | 1,28 | Yes | Yes |
| DPM14_19610 | Rv3574 | 4021857:4023419_forward | 1 | K | VAVLAESELGSEAQR | E | 46,2 | 25 | 46,2 | 2,6 | Yes | Yes |
| DPM14_19975 | Rv3646c | 4091010:4094006_reverse | 1 | R | PLPTGAVSIGAGIR | D | 0 | 0 | 0 | 1,08 | Yes | Yes |
| DPM14_20195 | Rv3684 | 4132188:4133507_forward | 2 | R | LIEADAR | R | 22,3 | 26,8 | 10,4 | 1,07 | Yes | Yes |
| R | SWTDNAIR | L | 24,9 | 25 | 9,15 | 0 | Yes | Yes | ||||
| DPM14_21155 | Rv3856c | 4335338:4336528_reverse | 1 | R | ATGVSPIMDPVTALR | Q | 14,4 | 25 | 13,1 | 1,11 | Yes | Yes |
| New genes | ||||||||||||
| NA | NA | 104947:105810_reverse | 2 | R | LVLRIELSGR | G | 0 | 0 | 0 | 1,59 | No | Yes |
| R | NDLSNYPAEFDELPR | R | 0 | 0 | 0 | 1,18 | Yes | Yes | ||||
| NA | NA | 1075075:1075662_reverse | 2 | R | EPVGVLGSGIGR | N | 20,3 | 25 | 15,4 | 0 | Yes | Yes |
| R | RGGGGVLR | G | 16,7 | 25 | −1,05 | 0 | Yes | Yes | ||||
| NA | NA | 1095047:1095517_reverse | 2 | R | IPQRCGVIR | N | 27,9 | 25 | 11,9 | 1,44 | Yes | No |
| R | IHQAPIRVLDR | E | 21,8 | 25 | 20,4 | 0 | Yes | No | ||||
| NA | NA | 1680143:1680568_reverse | 2 | - | NAVSSPGAGLATVAATR | T | 0 | 0 | 0 | 1,59 | Yes | No |
| R | PLNTMPLVR | T | 15,9 | 25 | 0 | 0 | Yes | No | ||||
| NA | NA | 1733676:1734110_reverse | 2 | R | PIPPIGALR | L | 24,9 | 25 | 0 | 0 | Yes | No |
| R | SGAASSGAR | P | 21,8 | 25 | 5,72 | 1,16 | Yes | No | ||||
| NA | NA | 1958092:1958697_forward | 2 | R | VAGPSNPELPGHPHPCR | G | 0 | 0 | 0 | 1,24 | Yes | No |
| R | DRPAGQPVR | R | 26,9 | 25,3 | 5,29 | 0 | Yes | No | ||||
| NA | NA | 2830724:2831131_forward | 2 | R | VHNLDPELVDEHAR | Q | 34,9 | 25 | 33,3 | 5,77 | Yes | No |
| R | YGPRPDPND | - | 0 | 0 | 0 | 1,57 | Yes | No | ||||
| NA | NA | 2911505:2912077_reverse | 2 | R | RSLLAMASPR | R | 37,4 | 25 | 10,8 | 0 | No | No |
| R | SLLAMASPR | R | 37,4 | 25 | 10,8 | 0 | No | No | ||||
| NA | NA | 3117184:3117807_reverse | 2 | R | IPSEVDGLLR | L | 22,7 | 25 | 17,2 | 0 | Yes | Yes |
| R | HAAQRVVGVAVFK | Q | 14,8 | 25 | 13,3 | 0 | Yes | Yes | ||||
| NA | NA | 3766655:3767689_forward | 2 | R | AGGQAQVGKPQVAMADVAQPQVAVPDIAQAQVVPTDVGR | T | 0 | 0 | 0 | 1,24 | Yes | No |
| R | VEATGVGVAGVEAARVVVTSVGDAGAEAAGIEEPGAGR | S | 0 | 0 | 0 | 2,28 | Yes | No | ||||
| NA | NA | 3999293:4001251_forward | 4 | R | GAGSIAGR | V | 30 | 25 | 14,8 | 0 | Yes | Yes |
| R | HPAQLLDLR | P | 26,5 | 25 | 21,5 | 0 | Yes | Yes | ||||
| R | KPIHADLR | Q | 28,3 | 25 | 21,5 | 0 | Yes | Yes | ||||
| R | HLHAVRAAGDIR | S | 14,8 | 25 | 14,8 | 0 | Yes | Yes | ||||