TONSIL-2.1 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.1 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.1 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.1 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.1 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.1 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.1 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
10 |
3 |
CD11c |
Alexa 488 |
FITC |
485 |
525 |
61 |
Cell Signaling Technology |
45581 S |
1:300 |
0.5 |
TONSIL-2.1 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.1 |
12 |
3 |
CD209 |
Alexa 647 |
Cy5 |
640 |
690 |
115 |
Santa Cruz |
sc-65740 |
1:100 |
0.5 |
TONSIL-2.1 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
14 |
4 |
CD11c |
Alexa 488 |
FITC |
485 |
525 |
61 |
Cell Signaling Technology |
45581 S |
1:300 |
0.2 |
TONSIL-2.1 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.1 |
16 |
4 |
CD209 |
Alexa 647 |
Cy5 |
640 |
690 |
115 |
Santa Cruz |
sc-65740 |
1:100 |
0.2 |
TONSIL-2.1 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
18 |
5 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:300 |
0.5 |
TONSIL-2.1 |
19 |
5 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.1 |
20 |
5 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.5 |
TONSIL-2.1 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
22 |
6 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.2 |
TONSIL-2.1 |
23 |
6 |
CD4 |
Alexa 555 |
Cy3 |
555 |
590 |
54 |
eBioscience |
41-2444-82 |
1:200 |
0.5 |
TONSIL-2.1 |
24 |
6 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.2 |
TONSIL-2.1 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
26 |
7 |
EGFR |
Alexa 488 |
FITC |
485 |
525 |
70 |
Cell Signaling Technology |
5616 S |
1:500 |
0.5 |
TONSIL-2.1 |
27 |
7 |
CD11c |
Alexa 555 |
Cy3 |
555 |
590 |
58 |
eBioscience |
41-9761-80 |
1:3'1:3'1:3 |
0.5 |
TONSIL-2.1 |
28 |
7 |
VIM |
Alexa 647 |
Cy5 |
640 |
690 |
40 |
BioLegend |
677807 |
1:500 |
0.1 |
TONSIL-2.1 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
30 |
8 |
IBA1 |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.1 |
31 |
8 |
CD86 |
Alexa 555 |
Cy3 |
555 |
590 |
14 |
BioLegend |
305405 |
1:100 |
0.5 |
TONSIL-2.1 |
32 |
8 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.1 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
34 |
9 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:300 |
0.5 |
TONSIL-2.1 |
35 |
9 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
36 |
9 |
CD64 |
Alexa 647 |
Cy5 |
640 |
690 |
11 |
BioLegend |
305012 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
38 |
10 |
CD19 |
Alexa 488 |
FITC |
485 |
525 |
6 |
BioLegend |
302219 |
1:200 |
0.5 |
TONSIL-2.1 |
39 |
10 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
40 |
10 |
CD134 |
Alexa 647 |
Cy5 |
640 |
690 |
23 |
BioLegend |
350018 |
1:300 |
0.5 |
TONSIL-2.1 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
42 |
11 |
IFNG |
Alexa 488 |
FITC |
485 |
525 |
33 |
BioLegend |
502517 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
43 |
11 |
PML |
Alexa 555 |
Cy3 |
555 |
590 |
100 |
Abcam |
ab217524 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
44 |
11 |
CD305 |
Alexa 647 |
Cy5 |
640 |
690 |
22 |
BioLegend |
342802 |
1:1'1:50 |
0.5 |
TONSIL-2.1 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.1 |
46 |
12 |
TIm3 |
Alexa 488 |
FITC |
485 |
525 |
69 |
Cell Signaling Technology |
54669 S |
1:200 |
0.5 |
TONSIL-2.1 |
47 |
12 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.1 |
TONSIL-2.1 |
48 |
12 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.2 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.2 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.2 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.2 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.2 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.2 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.2 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
10 |
3 |
CCR7 |
Alexa 488 |
FITC |
485 |
525 |
113 |
Invitrogen |
PA5-32299 |
1:100 |
0.5 |
TONSIL-2.2 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.2 |
12 |
3 |
CD45RO |
Alexa 647 |
Cy5 |
640 |
690 |
118 |
Dako |
M0742 |
1:300 |
0.5 |
TONSIL-2.2 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
14 |
4 |
CCR7 |
Alexa 488 |
FITC |
485 |
525 |
113 |
Invitrogen |
PA5-32299 |
1:100 |
0.2 |
TONSIL-2.2 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.2 |
16 |
4 |
CD45RO |
Alexa 647 |
Cy5 |
640 |
690 |
1118 |
Dako |
M0742 |
1:300 |
0.2 |
TONSIL-2.2 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
18 |
5 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:300 |
0.5 |
TONSIL-2.2 |
19 |
5 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.2 |
20 |
5 |
CD16 |
PacBlue |
Cy5 |
640 |
690 |
34 |
BD Biosiences |
558122 |
1:1'1:50 |
0.5 |
TONSIL-2.2 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
22 |
6 |
GATA3 |
Alexa 488 |
FITC |
485 |
525 |
35 |
BD Biosiences |
560163 |
1:100 |
0.5 |
TONSIL-2.2 |
23 |
6 |
EpCam |
Alexa 555 |
Cy3 |
555 |
590 |
19 |
BioLegend |
324205 |
1:300 |
0.5 |
TONSIL-2.2 |
24 |
6 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.2 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
26 |
7 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:500 |
0.5 |
TONSIL-2.2 |
27 |
7 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:2'1:50 |
0.5 |
TONSIL-2.2 |
28 |
7 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1'1:6'1:70 |
0.2 |
TONSIL-2.2 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
30 |
8 |
CD69 |
Alexa 488 |
FITC |
485 |
525 |
17 |
BioLegend |
310904 |
1:100 |
0.5 |
TONSIL-2.2 |
31 |
8 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.1 |
TONSIL-2.2 |
32 |
8 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.2 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
34 |
9 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.2 |
35 |
9 |
CD25 |
PE |
Cy3 |
555 |
590 |
28 |
BioLegend |
356104 |
1:1'1:50 |
0.5 |
TONSIL-2.2 |
36 |
9 |
CD86 |
Alexa 647 |
Cy5 |
640 |
690 |
15 |
BioLegend |
305416 |
1:1'1:50 |
0.5 |
TONSIL-2.2 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
38 |
10 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.2 |
TONSIL-2.2 |
39 |
10 |
CD45R |
Alexa 555 |
Cy3 |
555 |
590 |
53 |
Invitrogen |
41-0452-80 |
1:200 |
0.5 |
TONSIL-2.2 |
40 |
10 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.5 |
TONSIL-2.2 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
42 |
11 |
CD15 |
Alexa 488 |
FITC |
485 |
525 |
4 |
BioLegend |
301910 |
1:300 |
0.2 |
TONSIL-2.2 |
43 |
11 |
CD27 |
PE |
Cy3 |
555 |
590 |
29 |
BioLegend |
356406 |
1:1'1:50 |
0.2 |
TONSIL-2.2 |
44 |
11 |
PDL1 |
Alexa 647 |
Cy5 |
640 |
690 |
50 |
Cell Signaling Technology |
15005 S |
1:300 |
0.5 |
TONSIL-2.2 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.2 |
46 |
12 |
CD163 |
Alexa 488 |
FITC |
485 |
525 |
101 |
Abcam |
ab218293 |
1:300 |
0.5 |
TONSIL-2.2 |
47 |
12 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.2 |
48 |
12 |
HLA-A |
Alexa 647 |
Cy5 |
640 |
690 |
87 |
Abcam |
ab199837 |
1:200 |
0.2 |
TONSIL-2.3 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.3 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.3 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.3 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.3 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.3 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.3 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
10 |
3 |
CD115 |
Alexa 488 |
FITC |
485 |
525 |
77 |
Abcam |
ab183316 |
1:100 |
0.5 |
TONSIL-2.3 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.3 |
12 |
3 |
CD1a |
Alexa 555 |
Cy5 |
640 |
690 |
88 |
Abcam |
ab201337 |
1:30 |
0.5 |
TONSIL-2.3 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
14 |
4 |
CD115 |
Alexa 488 |
FITC |
485 |
525 |
77 |
Abcam |
ab183316 |
1:100 |
0.2 |
TONSIL-2.3 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.3 |
16 |
4 |
CD1a |
Alexa 647 |
Cy5 |
640 |
690 |
88 |
Abcam |
ab201337 |
1:30 |
0.2 |
TONSIL-2.3 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
18 |
5 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.5 |
TONSIL-2.3 |
19 |
5 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
20 |
5 |
IRF1 |
Alexa 647 |
Cy5 |
640 |
690 |
49 |
Cell Signaling Technology |
14105 S |
1:200 |
0.5 |
TONSIL-2.3 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
22 |
6 |
Lamin A/C |
Alexa 488 |
FITC |
485 |
525 |
73 |
Cell Signaling Technology |
8617 S |
1:300 |
0.5 |
TONSIL-2.3 |
23 |
6 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.1 |
TONSIL-2.3 |
24 |
6 |
CD3 |
Alexa 647 |
Cy5 |
640 |
690 |
63 |
eBioscience |
50-0037-41 |
1:300 |
0.5 |
TONSIL-2.3 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
26 |
7 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:500 |
0.5 |
TONSIL-2.3 |
27 |
7 |
Vinculin |
Alexa 555 |
Cy3 |
555 |
590 |
59 |
eBioscience |
41-9777-80 |
1:500 |
0.5 |
TONSIL-2.3 |
28 |
7 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1'1:6'1:70 |
0.2 |
TONSIL-2.3 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
30 |
8 |
CD49b |
Alexa 488 |
FITC |
485 |
525 |
30 |
BioLegend |
359305 |
1:100 |
0.5 |
TONSIL-2.3 |
31 |
8 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.3 |
32 |
8 |
IBA1 |
Alexa 647 |
Cy5 |
640 |
690 |
104 |
Bioss |
AIF1 |
1:100 |
0.5 |
TONSIL-2.3 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
34 |
9 |
TIM3 |
Alexa 488 |
FITC |
485 |
525 |
69 |
Cell Signaling Technology |
54669 S |
1:200 |
0.5 |
TONSIL-2.3 |
35 |
9 |
CD33 |
PE |
Cy3 |
555 |
590 |
31 |
BioLegend |
366608 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
36 |
9 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.3 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
38 |
10 |
CD28 |
Alexa 488 |
FITC |
485 |
525 |
8 |
BioLegend |
302954 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
39 |
10 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
40 |
10 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.3 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
42 |
11 |
PKR- |
Alexa 488 |
FITC |
485 |
525 |
102 |
Abcam |
ab219739 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
43 |
11 |
CD83 |
PE |
Cy3 |
555 |
590 |
13 |
BioLegend |
305308 |
1:1'1:50 |
0.5 |
TONSIL-2.3 |
44 |
11 |
CD206 |
Alexa 647 |
Cy5 |
640 |
690 |
18 |
BioLegend |
321116 |
1:100 |
0.5 |
TONSIL-2.3 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.3 |
46 |
12 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:300 |
0.5 |
TONSIL-2.3 |
47 |
12 |
CTLA4 |
PE |
Cy3 |
555 |
590 |
99 |
Abcam |
ab210254 |
1:200 |
0.5 |
TONSIL-2.3 |
48 |
12 |
CD16 |
APC |
Cy5 |
640 |
690 |
34 |
BD Biosiences |
558122 |
1:200 |
0.5 |
TONSIL-2.4 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.4 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.4 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.4 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.4 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.4 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.4 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
10 |
3 |
PDL2 |
Alexa 488 |
FITC |
485 |
525 |
71 |
Cell Signaling Technology |
82723 S |
1:200 |
0.5 |
TONSIL-2.4 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.4 |
12 |
3 |
p-cJun |
Alexa 647 |
Cy5 |
640 |
690 |
116 |
Santa Cruz |
sc-822 |
1:100 |
0.5 |
TONSIL-2.4 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
14 |
4 |
PDL2 |
Alexa 488 |
FITC |
485 |
525 |
71 |
Cell Signaling Technology |
82723 S |
1:200 |
0.2 |
TONSIL-2.4 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.4 |
16 |
4 |
p-cJun |
Alexa 647 |
Cy5 |
640 |
690 |
116 |
Santa Cruz |
sc-822 |
1:100 |
0.2 |
TONSIL-2.4 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
18 |
5 |
GATA3 |
Alexa 488 |
FITC |
485 |
525 |
35 |
BD Biosiences |
560163 |
1:200 |
0.5 |
TONSIL-2.4 |
19 |
5 |
CD66b |
Alexa 555 |
Cy3 |
555 |
590 |
43 |
Invitrogen |
12-0666-41 |
1:300 |
0.5 |
TONSIL-2.4 |
20 |
5 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.5 |
TONSIL-2.4 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
22 |
6 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
67 |
eBioscience |
53-0196-80 |
1:200 |
0.5 |
TONSIL-2.4 |
23 |
6 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.4 |
24 |
6 |
Her2 |
Alexa 647 |
Cy5 |
640 |
690 |
20 |
BioLegend |
324412 |
1:200 |
0.5 |
TONSIL-2.4 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
26 |
7 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1'1:6'1:70 |
0.2 |
TONSIL-2.4 |
27 |
7 |
CD133 |
Alexa 555 |
Cy3 |
555 |
590 |
45 |
eBioscience |
12-1338-41 |
1:3'1:3'1:3 |
0.5 |
TONSIL-2.4 |
28 |
7 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:3'1:3'1:3 |
0.5 |
TONSIL-2.4 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
30 |
8 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:300 |
0.5 |
TONSIL-2.4 |
31 |
8 |
CD31 |
Alexa 555 |
Cy3 |
555 |
590 |
107 |
R&D Systems |
FAB3567P |
1:100 |
0.5 |
TONSIL-2.4 |
32 |
8 |
CD103 |
Alexa 647 |
Cy5 |
640 |
690 |
24 |
BioLegend |
350209 |
1:100 |
0.5 |
TONSIL-2.4 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
34 |
9 |
CD15 |
Alexa 488 |
FITC |
485 |
525 |
4 |
BioLegend |
301910 |
1:1'1:50 |
0.2 |
TONSIL-2.4 |
35 |
9 |
CD80 |
PE |
Cy3 |
555 |
590 |
12 |
BioLegend |
305207 |
1:1'1:50 |
0.5 |
TONSIL-2.4 |
36 |
9 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.2 |
TONSIL-2.4 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
38 |
10 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:300 |
0.5 |
TONSIL-2.4 |
39 |
10 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.1 |
TONSIL-2.4 |
40 |
10 |
aSMA |
Alexa 647 |
Cy5 |
640 |
690 |
65 |
eBioscience |
50-9760-82 |
1:1000 |
0.2 |
TONSIL-2.4 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
42 |
11 |
TDP43 |
Alexa 488 |
FITC |
485 |
525 |
80 |
Abcam |
ab193842 |
1:1'1:50 |
0.5 |
TONSIL-2.4 |
43 |
11 |
FOXO1A |
Alexa 555 |
Cy3 |
555 |
590 |
95 |
Abcam |
ab207244 |
1:1'1:50 |
0.5 |
TONSIL-2.4 |
44 |
11 |
CD138 |
APC |
Cy5 |
640 |
690 |
26 |
BioLegend |
352308 |
1:1'1:50 |
0.5 |
TONSIL-2.4 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.4 |
46 |
12 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.4 |
47 |
12 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.4 |
48 |
12 |
CD16 |
Alexa 647 |
Cy5 |
640 |
690 |
114 |
Santa Cruz |
sc-20052 AF647 |
1:200 |
0.5 |
TONSIL-2.5 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.5 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.5 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.5 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.5 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.5 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.5 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
10 |
3 |
CD2 |
Alexa 488 |
FITC |
485 |
525 |
103 |
Abcam |
ab37212 |
1:100 |
0.5 |
TONSIL-2.5 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.5 |
12 |
3 |
GATA3 |
Alexa 647 |
Cy5 |
640 |
690 |
110 |
Thermo-Fisher |
MA1-028 |
1:100 |
0.5 |
TONSIL-2.5 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
14 |
4 |
CD2 |
Alexa 488 |
FITC |
485 |
525 |
103 |
Abcam |
ab37212 |
1:100 |
0.2 |
TONSIL-2.5 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.5 |
16 |
4 |
GATA3 |
Alexa 647 |
Cy5 |
640 |
690 |
110 |
Thermo-Fisher |
MA1-028 |
1:100 |
0.2 |
TONSIL-2.5 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
18 |
5 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.5 |
19 |
5 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.5 |
TONSIL-2.5 |
20 |
5 |
PDL1 |
Alexa 647 |
Cy5 |
640 |
690 |
50 |
Cell Signaling Technology |
15005 S |
1:300 |
0.5 |
TONSIL-2.5 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
22 |
6 |
CD11a |
Alexa 488 |
FITC |
485 |
525 |
41 |
eBioscience |
11-0119-41 |
1:200 |
0.5 |
TONSIL-2.5 |
23 |
6 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.5 |
24 |
6 |
PD1 |
Alexa 647 |
Cy5 |
640 |
690 |
89 |
abcam |
ab201825 |
1:200 |
0.5 |
TONSIL-2.5 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
26 |
7 |
GATA3 |
Alexa 488 |
FITC |
485 |
525 |
35 |
BD Biosiences |
560163 |
1:1'1:6'1:7 |
0.5 |
TONSIL-2.5 |
27 |
7 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1'1:6'1:7 |
0.5 |
TONSIL-2.5 |
28 |
7 |
Beta-catenin |
Alexa 647 |
Cy5 |
640 |
690 |
39 |
BioLegend |
658705 |
1:500 |
0.5 |
TONSIL-2.5 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
30 |
8 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.2 |
TONSIL-2.5 |
31 |
8 |
TIM1 |
Alexa 555 |
Cy3 |
555 |
590 |
27 |
BioLegend |
353904 |
1:100 |
0.5 |
TONSIL-2.5 |
32 |
8 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.2 |
TONSIL-2.5 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
34 |
9 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:300 |
0.5 |
TONSIL-2.5 |
35 |
9 |
aSMA |
Alexa 555 |
Cy3 |
555 |
590 |
90 |
Abcam |
ab202509 |
1:1000 |
0.1 |
TONSIL-2.5 |
36 |
9 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.5 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
38 |
10 |
CD19 |
Alexa 488 |
FITC |
485 |
525 |
84 |
Abcam |
ab196468 |
1:200 |
0.5 |
TONSIL-2.5 |
39 |
10 |
pH2AX |
Alexa 555 |
Cy3 |
555 |
590 |
36 |
BioLegend |
613412 |
1:300 |
0.2 |
TONSIL-2.5 |
40 |
10 |
RunX3 |
Alexa 647 |
Cy5 |
640 |
690 |
66 |
eBioscience |
50-9817-80 |
1:200 |
0.5 |
TONSIL-2.5 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
42 |
11 |
CD163 |
Alexa 488 |
FITC |
485 |
525 |
101 |
Abcam |
ab218293 |
1:300 |
0.5 |
TONSIL-2.5 |
43 |
11 |
CD66b |
Alexa 555 |
Cy3 |
555 |
590 |
43 |
Thermo-Fisher |
12-0666-41 |
1:100 |
0.5 |
TONSIL-2.5 |
44 |
11 |
Ki67 |
Alexa 647 |
Cy5 |
640 |
690 |
25 |
BioLegend |
350509 |
1:200 |
0.5 |
TONSIL-2.5 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.5 |
46 |
12 |
LaminA/C |
Alexa 488 |
FITC |
485 |
525 |
73 |
Cell Signaling Technology |
8617 S |
1:300 |
0.5 |
TONSIL-2.5 |
47 |
12 |
NFATc1 |
Alexa 555 |
Cy3 |
555 |
590 |
38 |
BioLegend |
649605 |
1:200 |
0.5 |
TONSIL-2.5 |
48 |
12 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.5 |
TONSIL-2.6 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.6 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.6 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.6 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.6 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.6 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.6 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
10 |
3 |
Axl |
Alexa 488 |
FITC |
485 |
525 |
74 |
Cell Signaling Technology |
8661 S |
1:300 |
0.5 |
TONSIL-2.6 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.6 |
12 |
3 |
IDO |
Alexa 647 |
Cy5 |
640 |
690 |
111 |
EMD-Millipore |
MAB10009 |
1:100 |
0.5 |
TONSIL-2.6 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
14 |
4 |
Axl |
Alexa 488 |
FITC |
485 |
525 |
74 |
Cell Signaling Technology |
8661 S |
1:300 |
0.2 |
TONSIL-2.6 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.6 |
16 |
4 |
IDO |
Alexa 647 |
Cy5 |
640 |
690 |
111 |
EMD-Millipore |
MAB10009 |
1:100 |
0.2 |
TONSIL-2.6 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
18 |
5 |
TIM3 |
Alexa 488 |
FITC |
485 |
525 |
69 |
Cell Signaling Technology |
54669 S |
1:200 |
0.5 |
TONSIL-2.6 |
19 |
5 |
Her2 |
Alexa 555 |
Cy3 |
555 |
590 |
57 |
eBioscience |
41-9757-80 |
1:300 |
0.5 |
TONSIL-2.6 |
20 |
5 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.6 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
22 |
6 |
CD123 |
Alexa 488 |
FITC |
485 |
525 |
16 |
BioLegend |
306035 |
1:200 |
0.5 |
TONSIL-2.6 |
23 |
6 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.6 |
24 |
6 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.2 |
TONSIL-2.6 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
26 |
7 |
CD163 |
Alexa 488 |
FITC |
485 |
525 |
101 |
Abcam |
ab218293 |
1:500 |
0.5 |
TONSIL-2.6 |
27 |
7 |
NFATc1 |
Alexa 555 |
Cy3 |
555 |
590 |
38 |
BioLegend |
649605 |
1:3'1:3'1:3 |
0.5 |
TONSIL-2.6 |
28 |
7 |
ABCC1 |
Alexa 647 |
Cy5 |
640 |
690 |
32 |
BioLegend |
370203 |
1:500 |
0.5 |
TONSIL-2.6 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
30 |
8 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:300 |
0.5 |
TONSIL-2.6 |
31 |
8 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.6 |
32 |
8 |
IBA1 |
Alexa 647 |
Cy5 |
640 |
690 |
104 |
Bioss |
AIF1 |
1:100 |
0.5 |
TONSIL-2.6 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
34 |
9 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
67 |
eBioscience |
53-0196-80 |
1:200 |
0.5 |
TONSIL-2.6 |
35 |
9 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.6 |
36 |
9 |
CD206 |
Alexa 647 |
Cy5 |
640 |
690 |
18 |
BioLegend |
321116 |
1:1'1:50 |
0.5 |
TONSIL-2.6 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
38 |
10 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.6 |
39 |
10 |
pH3 |
Alexa 555 |
Cy3 |
555 |
590 |
52 |
Cell Signaling Technology |
3475 S |
1:1000 |
0.1 |
TONSIL-2.6 |
40 |
10 |
Ki67 |
Alexa 647 |
Cy5 |
640 |
690 |
25 |
BioLegend |
350509 |
1:1000 |
0.5 |
TONSIL-2.6 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
42 |
11 |
GATA3 |
Alexa 488 |
FITC |
485 |
525 |
35 |
BD Biosiences |
560163 |
1:100 |
0.5 |
TONSIL-2.6 |
43 |
11 |
VEGFR2 |
PE |
Cy3 |
555 |
590 |
46 |
Cell Signaling Technology |
12634 S |
1:1'1:50 |
0.2 |
TONSIL-2.6 |
44 |
11 |
IRF1 |
Alexa 647 |
Cy5 |
640 |
690 |
49 |
Cell Signaling Technology |
14105 S |
1:200 |
0.5 |
TONSIL-2.6 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.6 |
46 |
12 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.2 |
TONSIL-2.6 |
47 |
12 |
CD11c |
Alexa 555 |
Cy3 |
555 |
590 |
58 |
eBioscience |
41-9761-80 |
1:200 |
0.5 |
TONSIL-2.6 |
48 |
12 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.7 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.7 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.7 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.7 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.7 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.7 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.7 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
10 |
3 |
STING |
Alexa 488 |
FITC |
485 |
525 |
48 |
Cell Signaling Technology |
13647 S |
1:100 |
0.5 |
TONSIL-2.7 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.7 |
12 |
3 |
CD1c |
Alexa 647 |
Cy5 |
640 |
690 |
75 |
Abcam |
ab156708 |
1:200 |
0.5 |
TONSIL-2.7 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
14 |
4 |
STING |
Alexa 488 |
FITC |
485 |
525 |
48 |
Cell Signaling Technology |
13647 S |
1:100 |
0.2 |
TONSIL-2.7 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.7 |
16 |
4 |
CD1c |
Alexa 647 |
Cy5 |
640 |
690 |
75 |
Abcam |
ab156708 |
1:200 |
0.2 |
TONSIL-2.7 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
18 |
5 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
67 |
eBioscience |
53-0196-80 |
1:300 |
0.5 |
TONSIL-2.7 |
19 |
5 |
CD45R |
Alexa 555 |
Cy3 |
555 |
590 |
53 |
invitrogen |
41-0452-80 |
1:300 |
0.5 |
TONSIL-2.7 |
20 |
5 |
CD25 |
Alexa 647 |
Cy5 |
640 |
690 |
7 |
BioLegend |
302617 |
1:1'1:50 |
0.5 |
TONSIL-2.7 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
22 |
6 |
CD163 |
Alexa 488 |
FITC |
485 |
525 |
101 |
Abcam |
ab218293 |
1:300 |
0.5 |
TONSIL-2.7 |
23 |
6 |
CD1c |
Alexa 555 |
Cy3 |
555 |
590 |
21 |
BioLegend |
331505 |
1:200 |
0.5 |
TONSIL-2.7 |
24 |
6 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
62 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.7 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
26 |
7 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:8'1:3'1:3 |
0.5 |
TONSIL-2.7 |
27 |
7 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1'1:6'1:70 |
0.1 |
TONSIL-2.7 |
28 |
7 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:500 |
0.5 |
TONSIL-2.7 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
30 |
8 |
BRD7 |
Alexa 488 |
FITC |
485 |
525 |
105 |
Aviva |
ARP39018-P050 |
1:100 |
0.5 |
TONSIL-2.7 |
31 |
8 |
Beta-Tubulin |
Alexa 555 |
Cy3 |
555 |
590 |
51 |
Cell Signaling Technology |
2116 S |
1:300 |
0.5 |
TONSIL-2.7 |
32 |
8 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.5 |
TONSIL-2.7 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
34 |
9 |
CD16 |
Alexa 488 |
FITC |
485 |
525 |
5 |
BioLegend |
302019 |
1:1'1:50 |
0.5 |
TONSIL-2.7 |
35 |
9 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.7 |
36 |
9 |
CD134 |
Alexa 647 |
Cy5 |
640 |
690 |
23 |
BioLegend |
350018 |
1:1'1:50 |
0.5 |
TONSIL-2.7 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
38 |
10 |
CD4 |
Alexa 488 |
FITC |
485 |
525 |
108 |
R&D Systems |
FAB8165G |
1:300 |
0.5 |
TONSIL-2.7 |
39 |
10 |
CD11c |
Alexa 555 |
Cy3 |
555 |
590 |
58 |
eBioscience |
41-9761-80 |
1:200 |
0.5 |
TONSIL-2.7 |
40 |
10 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.2 |
TONSIL-2.7 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
42 |
11 |
Fibronectin |
Alexa 488 |
FITC |
485 |
525 |
85 |
Abcam |
ab198933 |
1:1000 |
0.5 |
TONSIL-2.7 |
43 |
11 |
pAur |
Alexa 555 |
Cy3 |
555 |
590 |
47 |
Cell Signaling Technology |
13464 S |
1:200 |
0.5 |
TONSIL-2.7 |
44 |
11 |
STING |
Alexa 647 |
Cy5 |
640 |
690 |
86 |
Abcam |
ab198952 |
1:1'1:50 |
0.2 |
TONSIL-2.7 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.7 |
46 |
12 |
CD11b |
Alexa 488 |
FITC |
485 |
525 |
94 |
Abcam |
ab204271 |
1:300 |
0.5 |
TONSIL-2.7 |
47 |
12 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:1'1:50 |
0.5 |
TONSIL-2.7 |
48 |
12 |
PD1 |
Alexa 647 |
Cy5 |
640 |
690 |
89 |
Abcam |
ab201825 |
1:200 |
0.5 |
TONSIL-2.8 |
1 |
1 |
DAPI_1 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
2 |
1 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.5 |
TONSIL-2.8 |
3 |
1 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.8 |
4 |
1 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.5 |
TONSIL-2.8 |
5 |
2 |
DAPI_2 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
6 |
2 |
A488 background |
|
FITC |
485 |
525 |
|
|
|
|
0.2 |
TONSIL-2.8 |
7 |
2 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.8 |
8 |
2 |
A647 background |
|
Cy5 |
640 |
690 |
|
|
|
|
0.2 |
TONSIL-2.8 |
9 |
3 |
DAPI_3 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
10 |
3 |
CD69 |
Alexa 488 |
FITC |
485 |
525 |
92 |
Abcam |
ab202909 |
1:100 |
0.5 |
TONSIL-2.8 |
11 |
3 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.5 |
TONSIL-2.8 |
12 |
3 |
RORyT |
Alexa 647 |
Cy5 |
640 |
690 |
112 |
EMD-Millipore |
MABF81 |
1:50 |
0.5 |
TONSIL-2.8 |
13 |
4 |
DAPI_4 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
14 |
4 |
CD69 |
Alexa 488 |
FITC |
485 |
525 |
92 |
Abcam |
ab202909 |
1:100 |
0.2 |
TONSIL-2.8 |
15 |
4 |
A555 background |
|
Cy3 |
555 |
590 |
|
|
|
|
0.2 |
TONSIL-2.8 |
16 |
4 |
RORyT |
Alexa 647 |
Cy5 |
640 |
690 |
112 |
EMD-Millipore |
MABF81 |
1:50 |
0.2 |
TONSIL-2.8 |
17 |
5 |
DAPI_5 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
18 |
5 |
CD163 |
Alexa 488 |
FITC |
485 |
525 |
101 |
Abcam |
ab218293 |
1:300 |
0.5 |
TONSIL-2.8 |
19 |
5 |
CD11c |
Alexa 555 |
Cy3 |
555 |
590 |
58 |
eBioscience |
41-9761-80 |
1:200 |
0.5 |
TONSIL-2.8 |
20 |
5 |
CD45 |
Alexa 647 |
Cy5 |
640 |
690 |
10 |
BioLegend |
304056 |
1:300 |
0.5 |
TONSIL-2.8 |
21 |
6 |
DAPI_6 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
22 |
6 |
IBA |
Alexa 488 |
FITC |
485 |
525 |
82 |
Abcam |
ab195031 |
1:500 |
0.5 |
TONSIL-2.8 |
23 |
6 |
CD11a |
Alexa 555 |
Cy3 |
555 |
590 |
3 |
BioLegend |
301207 |
1:200 |
0.5 |
TONSIL-2.8 |
24 |
6 |
CD3 |
Alexa 647 |
Cy5 |
640 |
690 |
2 |
BioLegend |
300422 |
1:200 |
0.5 |
TONSIL-2.8 |
25 |
7 |
DAPI_7 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
26 |
7 |
TIM3 |
Alexa 488 |
FITC |
485 |
525 |
69 |
Cell Signaling Technology |
54669 S |
1:3'1:3'1:3 |
0.5 |
TONSIL-2.8 |
27 |
7 |
CD3D |
Alexa 555 |
Cy3 |
555 |
590 |
97 |
Abcam |
ab208514 |
1:2'1:50 |
0.5 |
TONSIL-2.8 |
28 |
7 |
PDL1 |
Alexa 647 |
Cy5 |
640 |
690 |
50 |
Cell Signaling Technology |
15005 S |
1:500 |
0.5 |
TONSIL-2.8 |
29 |
8 |
DAPI_8 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
30 |
8 |
GATA3 |
Alexa 488 |
FITC |
485 |
525 |
35 |
BD Biosiences |
560163 |
1:100 |
0.5 |
TONSIL-2.8 |
31 |
8 |
FoxP3 |
Alexa 555 |
Cy3 |
555 |
590 |
55 |
eBioscience |
41-4777-82 |
1:1'1:50 |
0.5 |
TONSIL-2.8 |
32 |
8 |
Annexin V |
Alexa 647 |
Cy5 |
640 |
690 |
37 |
BioLegend |
640911 |
1:1'1:50 |
0.5 |
TONSIL-2.8 |
33 |
9 |
DAPI_9 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
34 |
9 |
PCNA |
Alexa 488 |
FITC |
485 |
525 |
72 |
Cell Signaling Technology |
8580 S |
1:1000 |
0.2 |
TONSIL-2.8 |
35 |
9 |
Keratin |
Alexa 555 |
Cy3 |
555 |
590 |
56 |
eBioscience |
41-9003-82 |
1:1000 |
0.1 |
TONSIL-2.8 |
36 |
9 |
CD14 |
Alexa 647 |
Cy5 |
640 |
690 |
83 |
Abcam |
ab196169 |
1:1000 |
0.5 |
TONSIL-2.8 |
37 |
10 |
DAPI_10 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
38 |
10 |
CD38 |
Alexa 488 |
FITC |
485 |
525 |
9 |
BioLegend |
303511 |
1:1'1:50 |
0.5 |
TONSIL-2.8 |
39 |
10 |
CD68 |
Alexa 555 |
Cy3 |
555 |
590 |
1 |
Cell Signaling Technology |
79594 S |
1:1000 |
0.5 |
TONSIL-2.8 |
40 |
10 |
CD8a |
Alexa 647 |
Cy5 |
640 |
690 |
114 |
eBioscience |
50-0008-82 |
1:200 |
0.5 |
TONSIL-2.8 |
41 |
11 |
DAPI_11 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
42 |
11 |
S100a |
Alexa 488 |
FITC |
485 |
525 |
96 |
Abcam |
ab207367 |
1:1'1:50 |
0.1 |
TONSIL-2.8 |
43 |
11 |
SQSTM1 |
Alexa 555 |
Cy3 |
555 |
590 |
93 |
Abcam |
ab203430 |
1:1'1:50 |
0.5 |
TONSIL-2.8 |
44 |
11 |
Ki67 |
Alexa 647 |
Cy5 |
640 |
690 |
44 |
Cell Signaling Technology |
12075 S |
1:300 |
0.05 |
TONSIL-2.8 |
45 |
12 |
DAPI_12 |
Hoechst 33342 |
DAPI |
395 |
431 |
119 |
Cell Signaling Technology |
4082 S |
1:1000 |
0.03 |
TONSIL-2.8 |
46 |
12 |
EGFR |
Alexa 488 |
FITC |
485 |
525 |
70 |
Cell Signaling Technology |
5616 S |
1:500 |
0.5 |
TONSIL-2.8 |
47 |
12 |
aSMA |
Alexa 555 |
Cy3 |
555 |
590 |
90 |
Abcam |
ab202509 |
1:1000 |
0.1 |
TONSIL-2.8 |
48 |
12 |
CD20 |
Alexa 647 |
Cy5 |
640 |
690 |
64 |
eBioscience |
50-0202-80 |
1:1000 |
0.2 |