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. 2019 Dec 17;10:5520. doi: 10.1038/s41467-019-13549-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Streptococcus pneumoniae consensus genome reconstructed from metagenomic sequences recovered from the ancient pitch. From outer to inner ring: S. pneumoniae virulence genes (black, shared genes are shown in bold); S. pneumoniae coding regions on the positive (blue) and negative (red) strand; mappability (grey); sequence depth for the Syltholm pitch (orange), HOMP sample SRS014468 (light brown), SRS019120 (light blue), SRS013942 (turquoise), SRS015055 (blue), and SRS014692 (dark blue). Sequence depths were calculated by aligning to the S. pneumoniae TIGR4 reference genome and visualised in 100 bp windows using Circos⁷³.