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. 2019 Dec 17;9:19226. doi: 10.1038/s41598-019-55429-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assay of transcriptional activation for GSF. (A) Various forms of GSF, full-length (GSF^1~557, positions 1–557), truncated transmembrane (GSF^1–521, positions 1–521), truncated C terminal half (GSF^1–160, positions 1–160) and truncated N terminal half (GSF^161–521, positions 161–521), fused with Gal4 DNA-binding domain (GAL4BD) to form four different constructs (GAL4BD-GSF^1~557, GAL4BD-GSF^1~521, GAL4BD-GSF^1~160 and GAL4BD-GSF^161~521). GAL4BD-GAL4AD is a positive control, while GAL4BD only served as a negative control. (B) The six constructs from (A) were introduced into the yeast strain AH109 and grown on SD without tryptophan (W⁻) or lacking tryptophan, histidine and adenine (WHA⁻). (C) Quantification of the β-galactosidase activity for six different transformants in yeast from (B).