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. 2019 Dec 17;10:5746. doi: 10.1038/s41467-019-13758-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Depiction of the general pathway that leads to PTI response. b List of A. thaliana plants used in this experiment. Fluorescence and brightfield imaging (×40 magnification) of epidermal peels applied with AEP (100 µM) for wild-type Col-0 c, PRR-deficient fls2/efr/cerk1 d, and the ethylene biosynthesis defected acs1/2/6/4/5/9/7/11 e. Additionally, supplementation studies were performed using the PAMPs, flg22, and elf18 (4.8 µM), which were applied to all plants. f Summary of the measured fluorescence between the various conditions studied. Statistical analysis was performed using a one-way ANOVA with Tukey’s multiple comparisons test. If not indicated, comparisons were made between the mutants and wild-type under the same treatment conditions. *P < 0.03, **P < 0.002, ***P < 0.0002, ****P < 0.0001, ns = not significant. Error is represented as s.d. of three independent experiments. Source data are provided as a Source Data file.