Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 17;9:19277. doi: 10.1038/s41598-019-55683-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Quantification of GIRK1 mRNA and protein in the different cell lines. (A) GIRK1 mRNA levels normalized to MCF10A^WT. WT, eGFP and GIRK1 denote wildtype, eGFP (control) and GIRK1 overexpressors, respectively. The median value is represented by the black line within the box, box margins represent 75% and 25% percentiles, whiskers indicate 90% and 10% percentiles. The red line represents the mean value. Individual values are shown as grey circles. The number of individual experiments is given in parenthesis above each box. Statistically significant differences between groups are indicated above brackets (n.s.: statistically not significant). (B) WB analysis of cell lysates: upper panel: MCF10A lysates (3 µg protein per slot was applied) probed with GIRK1^CT Ab. #1: MCF10A^WT, #2: MCF10A^eGFP and #3: MCF10A^GIRK1. Lower panel: MCF7 lysates (3 µg protein per slot) probed with GIRK1^CT Ab. #1: MCF7^WT, #2: MCF7^eGFP and #3: MCF7^GIRK1. (C)WB analysis of immunoprecipitates derived using GIRK1^CT Ab on MCF7^WT cell lysates. Left: membrane probed using GIRK1^CT Ab. #a1: lysate (30 µl), #a2: IP (30 µl). Middle: #b1: IP (30 µl) membrane probed using GIRK1^NT Ab (N-terminal) Ab. Right: #c1: IP (30 µl). Membrane probed using monoclonal GIRK1 Ab.