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. 2019 Dec 17;9:19277. doi: 10.1038/s41598-019-55683-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of MEC lines in cell culture. (A) Representative original results from cell cycle assessment by gated cell sorting according to fluorescence intensities (x-axis: PerCP-A; y-axis: PacificBlue). Left: MCF10A^eGFP (top) and MCF10^GIRK1 (bottom). Right: MCF7^eGFP (top) and MCF7^GIRK1 (bottom). Individual cells (represented as dots), attributed to a particular phase of the cell cycle are surrounded by black line (green dots: G₀/G₁ phase; purple dots: G₂/M phase and blue dots: S phase). (B) Statistical analysis of the percentage of time spend at different stages of the cell cycle. Left: MCF10A^WT, MCF10A^eGFP and MCF10A^GIRK1. Right: MCF7^WT, MCF7^eGFP and MCF7^GIRK1. The differences between WT, eGFP and GIRK1 do not differ statistically significant for either MCF10A nor MCF7.