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. 2019 Dec 17;9:19277. doi: 10.1038/s41598-019-55683-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular migration of MCF10A cells. (A) Migration of 5 selected MCF10A^GIRK1 cells over the entire observation interval. Left: flower plots showing cellular trajectories. Starting position of each individual cell was set to the same position, indicated by grey circle. Colored circle indicates the positon of a cell after 72 h. Right: squared distance as a function of time for the five cells shown to the left (circles; bars indicate standard error). Lines represent linear fits through the data. (B) Same as (A), but MCF10A^eGFP. (C) Statistical analysis of motility coefficients derived from the different experimental groups. WT: MCF10A^WT, eGFP: MCF10A^eGFP and GIRK1: MCF10A^GIRK1. The median value is represented by the black line within the box, box margins represent 75% and 25% percentiles, whiskers indicate 90% and 10% percentiles. The red line represents the mean value. Individual values are shown as dots. The number of individual cells is given in parenthesis besides each box. Statistically significant differences between groups are indicated by brackets.