Figure 6.

Aberrant nucleosome retention in Gcn5^cKO sperm. A) Number of evicted (grey), retained from any previous stage (orange), or newly accessible (blue) nucleosomes in Gcn5^Ctrl or Gcn5^cKO Sp. B) Compartment analysis of nucleosome location based on percent of total nucleosomes (green bar, genomic background). C) Top enriched GO terms for aberrantly retained Gcn5^cKO nucleosomes (number of genes in parentheses). Alluvial plot of nucleosomes in open (detected in specific cell type, yellow bar) or closed (not detected, grey) nucleosomes in (D) Gcn5^Ctrl or (E) Gcn5^cKO. Ribbon colors as described above. F) Number of evicted nucleosome dense regions with H3K9ac (defined by ChIP-seq, Bryant et. al., 2015) in RS. G) Enriched TF binding motifs present in newly open subnucleosomal regions in Gcn5^cKO Sp. H) Donut plot depicting changes in REs from total ATAC-seq signal for Gcn5^Ctrl (top) or Gcn5^cKO (bottom). Plots as described above. F) Percentage of nucleosomes retained in Sp overlapping with nucleosomes in pre-implantation embryos. E. 2-cell: early 2-cell, ICM: inner cell mass. See also Figure S5.