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NHQM-mediated protein cross-linking in vitro. A) Scheme showing NHQM structure and photo-controlled cross-linking mechanism. Upon UV activation, a highly reactive ortho-QM was generated capable of reacting with multiple nucleophilic residues. B) Protein 14-3-3 forms a homodimer (PDB code: 4N7Y). C-D) SDS-PAGE analysis of NHQM-mediated dimeric cross-linking of 14-3-3 (C) with different UV exposure time (D).
