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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Nov 8;58(52):18839–18843. doi: 10.1002/anie.201910135

Figure 2.

Figure 2.

Facile differentiation of dimer and monomer via NHQM cross-linking in vitro and in cell lysate. A-B) NHQM should cross-link the WT 14-3-3 dimer into a covalent dimeric form but not the monomeric QQR mutant, which can be readily distinguished on SDS-PAGE or Western blot. C-D) SDS-PAGE analysis of NHQM-mediated in vitro cross-linking WT 14-3-3 (C) and 14-3-3(QQR) mutant (D). E-F) Western blot analysis of NHQM-mediated cross-linking of WT 14-3-3 (E) and 14-3-3(QQR) mutant (F) in E. coli cell lysate. 14-3-3 was detected by an anti-Hisx6 antibody.