Figure 1:

Microglia from head-shielded bone marrow chimeric mice are distinct from infiltrating macrophages after TBI. Brains isolated from chimeric mice post TBI were analyzed by flow cytometry. CD45.1^neg ‘B, T, NK cells and Eosinophils’ acted as the gating control for CD45.2^hi cells, showing that resident microglia (CD45^lo) can be unambiguously differentiated from infiltrating monocyte-derived macrophages (CD45.1⁺). Arrows indicate the directionality of gating.