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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Surg Res. 2019 Sep 26;246:113–122. doi: 10.1016/j.jss.2019.08.024

Table I.

List of antibody conjugated fluorochromes used to differentiate microglia from infiltrating leukocytes.

Antibody Fluorochrome Company and Clone
CD45.1 FITC A-20 / BD Bioscience
CD45.2 BV421 104 / Biolegend
CD64 APC X54-5/7.1/ BD Bioscience
CD11b APC- Cy7 M1/70 / BD Biosciences
CD11c PE-Cy7 HL3 / BD Bioscience
Ly6G Alexa Flour 700 1A8 / BD Bioscience
MHC II Percp- Cy 5.5 M5/114.15.2 / Biolegend
Siglec H PE 551 / Biolegend
B220 PECF594 RA3-6B2 / BD Bioscience
Siglec F PECF594 E50-2440 / BD Bioscience
CD4 PECF594 RM4-5/ BD Bioscience
CD8 PECF594 53-6.7 / BD Bioscience
NK1.1 PECF594 PK136/ BD Bioscience
Viability e-bioscience Fixable Viability Dye eFluor 506 Invitrogen