Table I.
Antibody | Fluorochrome | Company and Clone |
---|---|---|
CD45.1 | FITC | A-20 / BD Bioscience |
CD45.2 | BV421 | 104 / Biolegend |
CD64 | APC | X54-5/7.1/ BD Bioscience |
CD11b | APC- Cy7 | M1/70 / BD Biosciences |
CD11c | PE-Cy7 | HL3 / BD Bioscience |
Ly6G | Alexa Flour 700 | 1A8 / BD Bioscience |
MHC II | Percp- Cy 5.5 | M5/114.15.2 / Biolegend |
Siglec H | PE | 551 / Biolegend |
B220 | PECF594 | RA3-6B2 / BD Bioscience |
Siglec F | PECF594 | E50-2440 / BD Bioscience |
CD4 | PECF594 | RM4-5/ BD Bioscience |
CD8 | PECF594 | 53-6.7 / BD Bioscience |
NK1.1 | PECF594 | PK136/ BD Bioscience |
Viability | e-bioscience Fixable Viability Dye eFluor 506 | Invitrogen |