Characterization of mouse ASCs and ASC EVs. (A) Representative immunofluorescence images of mouse ASCs stained with antibodies specific for the mesenchymal stem cell markers CD29, CD44, and CD90, respectively. Scale bar, 50 μm. (B) Representative transmission electron microscopy images of mouse ASC EVs released by naïve (EV) and IFNγ-primed ASCs (iEV) along with the EV-free EV collection medium. (C) Western blot detects exosome markers CD9 and CD63 in isolated ASC EVs. STD, size standard. MW, molecular weight.