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. Author manuscript; available in PMC: 2020 Dec 16.
Published in final edited form as: Curr Biol. 2019 Nov 27;29(24):4291–4299.e4. doi: 10.1016/j.cub.2019.10.027

Figure 3. Effects of chemogenetic manipulation of the opioid system on body temperature.

Figure 3.

Change in Tb response upon chemogenetic activation (hM3Dq) of Pdyn-expressing neurons in the ARC (A) or PBN (C) during ad libitum feeding. (E) Change in Tb response upon chemogenetic activation (hM3Dq) or inhibition (hM4Di) of Oprk-expressing neurons in the POA during ad libitum. (G) Change in Tb response upon chemogenetic inhibition of Oprk-expressing neurons in the POA during CR. (A, C, E and G) n = 6 mice per treatment; graphs show mean ± SEM. Two-way RM ANOVA followed by Sidak’s or Dunnett’s multiple comparisons test. (B, D, F and H) Representative images showing viral placement for each targeted region are shown. SCP, superior cerebellar peduncle; AC, anterior commissure; 3V, 3rd ventricle. (A) F(1, 10)=6.365 P =0.0302 between treated groups; Sidak’s *P < 0.05. (C) F(1, 10)=7.247, P =0.0226 between treated groups; Sidak’s *P < 0.05. (E) F (2, 15) = 6.099 P = 0.01; Dunnett’s *P < 0.05, baseline vs hM3Dq. (G) F(1, 10)=11.22, P =0.0074 between treated groups; Sidak’s *P < 0.05. Dippa Hydrochloride did not alter Tb in animals fed ad libitum (Figure S2).