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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Hepatology. 2019 Aug 9;71(1):14–30. doi: 10.1002/hep.30815

Fig. 2. Quantification of infection in HBV, HDV, and co-infected SACC-PHHs by HDV vPLAYR/TSA and anti-HBcAg immunofluorescence staining.

Fig. 2.

SACC-PHHs were either infected with HBV (MOI=4,000), HDV (MOI=1,000), or both HBV/HDV (HBV MOI=4,000, HDV MOI=1,000). (A) At 8 (top) and 28 (bottom) days post infection, control and infected SACC-PHHs were fixed and stained for HDV genomic RNA (green) by a vPLAYR/TSA procedure and for HBcAg (red) by an anti-HBcAg antibody as well as with DAPI (blue) for nuclear DNA. Quantification of three different images for each experimental condition were performed (approximately ~800 cells total per condition) for (B) HBcAg positive cells; (C) HDV genomic RNA positive cells; and (D) dual-positive cells.