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. 2019 Dec 17;10(6):e02867-19. doi: 10.1128/mBio.02867-19

FIG 2.

FIG 2

Effect of GPS1 downregulation on virus replication. (A) Downregulation of GPS1 expression by siRNA for GPS1 was examined by Western blotting (WB). (B) A cell viability assay was performed on the siRNA-treated cells. Cell viability was assessed by measuring the amount of ATP in each siRNA-transfected cell. The experiments were performed in triplicate, and the error bars represent the standard deviation for triplicate samples. Statistical analysis was carried out by using analysis of variance (ANOVA), followed by Dunnett’s test. *, P < 0.05 for three independent experiments. (C) Virus titers in the supernatants of siRNA-transfected cells. The experiments were performed in triplicate, and the error bars represent the standard deviation for triplicate samples. Statistical analysis was carried out by using ANOVA, followed by Dunnett’s test. ***, P < 0.001 for three independent experiments. (D) The degree of GPS1 downregulation was assessed by using four different siRNAs for GPS1 (siRNA GPS1_2, siRNA GPS1_3, siRNA GPS1_5, and siRNA GPS1_6). GPS1 was detected by Western blotting using the anti-GPS1 antibody. (E) The virus titers in the supernatants of the siRNA-treated cells were examined by using plaque assays at 48 hpi. The experiments were performed in triplicate; the error bars represent the standard deviation for triplicate samples. Statistical analysis was carried out by using ANOVA, followed by Dunnett’s test. *, P < 0.05 for three independent experiments.