FIG 5.

Effect of GPS1 downregulation on influenza virus protein and RNA expression. (A) Effect of GPS1 downregulation on viral protein expression. The expression levels of viral proteins in virus-infected cells were examined. siRNA-treated HEK293 cells were infected with virus at an MOI of 10. The cells were lysed at 3, 6, 9, and 12 hpi, and viral proteins were subjected to Western blotting. (B to D) Effect of GPS1 downregulation on viral RNA expression. The kinetics of the synthesis of the vRNA (B), cRNA (C), and mRNA (D) of the NP segment were examined by quantitative real-time PCR. The experiments were performed in triplicate; error bars represent the standard deviation for triplicate samples. Statistical analysis was carried out by using two-way ANOVA, followed by Sidak’s multiple-comparison test. ***, P < 0.001 for three independent experiments.