FIG 5.

Vaccination with CPS2 alters the host transcriptome during infection with ST258. (A) Three separate lung tissue samples were obtained from each animal at necropsy and processed individually for analysis by RNA-seq as described in the Methods and Materials. Results are presented as the mean fold change in transcripts from 3 to 6 separate samples from vaccinated versus sham-vaccinated animals. (B) RNA-seq data were analyzed with Ingenuity Pathway Analysis (IPA, Qiagen) to identify processes that are predicted to be increased or decreased. The intensity of orange or blue color indicates prediction strength. Increases or decreases that are ≤−2 or ≥ 2 indicate the change in predicted function is statistically significant.