Figure 7.

Glutathione degradation varies in steatotic livers during SNMP compared to NMP. (a) The glutathione cycle involves the degradation of glutathione into its base amino acids. Synthesis of glutathione involves three molecules of ATP. (b) Heatmap of the metabolites in the glutathione cycle compared between SNMP and NMP of steatotic livers. There is a significant depletion of the precursor metabolites in the synthesis arm of the cycle during SNMP compared to NMP, as well as a larger accumulation of 5-oxoproline. Reduced glutathione content in hepatocytes is decreased during NMP but depletion is near-total during SNMP. GSH, reduced glutathione; GSSH, oxidized glutathione; Cys-Gly, cysteinylglycine; γ-Glu-Cys, γ-glutamylcysteine; NMP, normothermic machine perfusion; SNMP, subnormothermic machine perfusion; x-axis represents fold change at 60, 120, and 180 min compared to pre-perfusion concentrations.