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. 2019 Oct 28;9(11):252. doi: 10.3390/metabo9110252

Figure 5.

Figure 5

Diet effects on oxylipins derived from ω6 polyunsaturated fatty acids (PUFA). (A,D): Hepatic content of linoleic acid (LA; 18:2, ω6) and arachidonic acid (ARA; 20:4, ω6) as determined by gas chromatographic (GC) analysis. Results are presented as nmoles/mg protein, mean ± SEM. Oxylipins derived from LA (B) and ARA (C) were quantified, by HPLC-dMRM as described in the Materials and Methods Section. Liver samples were derived from the RD group and oxylipin levels are presented as the peak area/mg protein, mean ± SEM. Effects of diet on specific oxylipins are presented in (Panels E,F) as Fold Change, mean ± SEM. (Panels E,F) present LA- and ARA-derived oxylipins, respectively. *, q < 0.05 vs. RD30; #, q < 0.05 vs. WDO30. 9(S)-HODE, 9(S) hydroxyl octadecadienoic acid; 13(S)-HODE, 13(S) hydroxyl octadecadienoic acid; 12,13-DiHOME, 12,13-dihydroxy octadecenoic acid; 6-keto PGF1α, 6-keto prostaglandin F1α; TbxB2, thromboxane B2; PGD2, prostaglandin D2; PGE2, prostaglandin E2, 5-HETE, 5-hydroxyeicosatrienoic acid; 12-HETE, 12-hydroxyeicosatrienoic acid; 15-HETE, 15-hydroxyeicosatrienoic acid; 20-HETE, 20-hydroxyeicosatrienoic acid; 11,12-EpETrE, 11,12 epoxyeicosatrienoic acid; 14,15-EpETrE, 14,15 epoxyeicosatrienoic acid; 14,15-diHETrE, 14,15-dihydroxyeicosatrienoic acid.