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. 2019 Dec 16;3(1):e201900424. doi: 10.26508/lsa.201900424

Figure 1. aYAP modRNA expression in the myocardium.

Figure 1.

(A) aYAP modRNA–transduced cardiomyocytes. 16 h after aYAP modRNA transfection, NRVMs were fixed for immunofluorescence staining. Bar = 50 µm. (B) YAP mRNA level measured by qRT-PCR. *P < 0.05, n = 3. Hearts were collected 2 d after IR and modRNA injection. (C) Immunoblot to show the expression of YAP protein. aYAP modRNA was fused to a 3×Flag tag. Flag antibody immunoblot showed the expression of aYAP in the aYAP modRNA–treated group but not in the vehicle-treated group. (D) Immunohistochemistry staining of Flag-YAP one day (D1), two days (D2) and 1 mo after IR. Bar = 500 µm. (E, F) Immunofluorescence staining of Flag-YAP. Cardiac troponin I (TNNI3) was used to label cardiomyocytes. (E) Low magnification of vehicle or aYAP-transduced myocardium. Bar = 50 µm. (F) high magnification of aYAP-transduced cardiomyocytes. Bar = 25 µm.