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. 2019 Dec 16;3(1):e201900424. doi: 10.26508/lsa.201900424

Figure 2. aYAP modRNA increases CM survival.

Figure 2.

(A) Experimental design. IR and modRNA injection was performed as described in the Materials and Methods section. Red fluorescence beads were injected into the left ventricle cavity after LAD ligation to label blood perfused area. 1 d after IR, EdU and MF20 antibodies were intraperitoneally injected to label CMs proliferation and necrosis, respectively. At day 2 after IR, hearts were collected for histology analysis. (B) MF20 staining of heart cross sections. Bar = 500 µm. (C) Quantification of myocardium with MF20 signals. t test: *P < 0.05, n = 4. (D) Triphenyltetrazolium chloride staining of heart cross sections 2 d after IR. Bar = 200 µm. (E) Quantification of myocardial scar size. t test: *P < 0.05, n = 4. (F) Peripheral blood serum troponin T concentration at different days after IR and aYAP modRNA treatment. Sham n = 3; Veh+IR, n = 4; aYAP+IR. t test: *P < 0.05.