Table 3. Summary of results of laboratories in the external quality assesment on molecular diagnostics of emerging neurotropic viruses, Europe (n = 51) .
| Sample ID | 1a | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| Virus | TOSV (lineage A) | TOSV (lineage B) | USUV | WNV (lineage 1) | WNV (lineage 2) | TBEV | Negative | Negative | Negative | Negative |
| Concentration | 1.57 x 105 RNA cp/0.4mL | 1.24 x 105 RNA cp/0.4mL | 6.34 x 103 RNA cp/0.4mL | 7.2 x 104 RNA cp/0.4mL | 4.96 x 105 RNA cp/0.4mL | 5.06 x 104 RNA cp/0.4mL | n/a | n/a | n/a | n/a |
| Total correct positive when tested for the specific virus | 31/51 | 28/51 | 23/51 | 42/51 | 46/51 | 37/51 | n/a | n/a | n/a | n/a |
| Total correct positive when not tested for the specific virus | 17/51 | 18/51 | 19/51 | 2/51 | 2/51 | 8/51 | n/a | n/a | n/a | n/a |
| Total correct | 48/51 | 46/51 | 42/51 | 44/51 | 48/51 | 45/51 | 47/51 | 48/51 | 49/51 | 48/51 |
| Total partially correct: identification at the genus level | 0/51 | 0/51 | 1/51 | 2/51 | 1/51 | 2/51 | n/a | n/a | n/a | n/a |
| False | 4/51 | 5/51 | 5/51 | 5/51 | 2/51 | 4/51 | 3/51 | 3/51 | 1/51 | 3/51 |
| Total sentivity | 31/32 | 28/32 | 21/28 | 42/48 | 46/48 | 36/42 | n/a | n/a | n/a | n/a |
n/a: not applicable; TBEV: tick-borne encephalitis virus; TOSV: Toscana virus; USUV: Usutu virus; WNV: West Nile virus.
a Number > 100% as one laboratory submitted both a correct result (positive for TOSV) and one false result (positive for TBEV) for this sample.