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. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Curr Biol. 2003 Jul 1;13(13):1106–1115. doi: 10.1016/s0960-9822(03)00418-4

Figure 5. Rnd proteins activate p190 molecules in vitro.

Figure 5.

(A) In vitro GAP assays using fluorophore-labeled PBP to detect GTP hydrolysis. PBP and GTP-loaded RhoA was mixed with GST,GST-Rnd3, or GST-Rnd3(T55A) in the absence or presence of purified p190A (A), and the rates of RhoA GTP hydrolysis were measured as described in Experimental Procedures.

(B) Activation of p190A- or p190B-mediated RhoA GTP hydrolysis by addition of GST-Rnd1, GST-Rnd2, or GST-Rnd3 (calculated as described in Experimental Procedures). The data shown represent the mean and SD of three independent experiments.