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. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Curr Biol. 2003 Jul 1;13(13):1106–1115. doi: 10.1016/s0960-9822(03)00418-4

Figure 6. Rnd proteins activate p190 molecules in cells.

Figure 6.

(A) Rhotekin RBD pulldown assay to determine levels of GTP-bound RhoA in control (p190A/B+/+), p190A−/−, and p190B−/− cells following transduction of Tat-HA-Rnd3 or Tat-HA-Rnd3(T55A). GTP-bound RhoA (RBD) and total RhoA in 2% of cell lysate (Lysates) were determined by western blot analysis using an antibody specific for RhoA. Protein transduction efficiency was confirmed with anti-HA antibody to detect Tat-HA-Rnd3 protein. The histogram shows RhoA-GTP levels in Tat-HA-Rnd3 and Tat-HA-Rnd3(T55A) transduced control, p190A−/−, and p190B−/− cells expressed as a ratio of RhoA-GTP levels in the corresponding untransduced cell lines. The data represent the mean and SD of three independent experiments.

(B–E) Morphological effects of transduction of Tat-HA-Rnd3 (B, D, E) or Tat-HA-Rnd3(T55A) (C) into control cells (B and C) or cells deficient in p190A (D) or p190B (E).