Fig. 5 |. Effect of RAD52 and dn53BP1 on microhomology distribution at the target site.
a,b, Microhomology distribution at the junctions with respect to RAG1 bait at the on-target sites for gCCR5D (a) and gCCR5Q (b) in the presence of RAD52 and/or dn53BP1. The number of junctions with <11-bp microhomology analysed for each condition is shown in brackets. CR, Cas9 + gRNA. Pooled data from three independent experiments normalized to 22,086 total junctions for each condition are shown. Error bars represent s.e.m. Significance was calculated using two-way analysis of variance (*P < 0.05, **P < 0.01, ***P < 0.0001).