Table 1 |.
HTGTS analysis of on- and off-target sites associated with gRNAs targeting CCR5
| Chromosome | Start | End | Target | Sequence | PAM | Total junctions |
Frequency (10,000 junctions) |
|---|---|---|---|---|---|---|---|
| chr3 | 46,411,328 | 46,420,240 | CCR5_D | TCACTATGCTGCCGCCCAGT | (GGG) | 1,619 | 307.1 |
| chr3 | 46,412,265 | 46,420,077 | CCR5_Q | GCTGTGTTTGCGTCTCTCCC | (AGG) | 1,222 | 153.4 |
| chr1 | 109,834,518 | 109,843,369 | Q_OT1 | GCTGTGTCCGCGCCTCTCCC | (CGG) | 1,932 | 242.5 |
| chr20 | 23,301,706 | 23,302,705 | Q_OT2 | GCTGTGTTTGCGCCTTTCCC | (AGG) | 58 | 7.3 |
| chr11 | 62,782,827 | 62,783,824 | Q_OT3 | GCTGTGTTTGTGCCTCCCCC | (AGG) | 11 | 1.4 |
| chr12 | 120,933,483 | 120,934,463 | Q_OT4 | GCTGTGCTAGCACCTCCCCC | (AGG) | 9 | 1.1 |
| chr10 | 77,357,580 | 77,358,098 | Q_OT5 | GCTGTGTGCTCGTCTCTCCC | (TGG) | 8 | 1 |
| chrX | 45,602,391 | 45,603,389 | Q_OT6 | TGGGTGTCTGCAGCTCTCCC | (AGG) | 8 | 1 |
| chr11 | 134,037,226 | 134,037,726 | Q_OT7 | CACGAGTGTGCGCCTCCCCC | (AGG) | 6 | 0.8 |
Pooled data from three independent experiments are shown. On-target chromosomal translocations are shown in bold. Off-target (OT) mismatches are underlined. The numbers of translocation junctions analysed were n = 52,719 for gCCR5D and n = 79,677 for gCCR5Q.