Table 1.
Primer Pairs Used for Real-Time PCR
| Gene | Forward and reverse primers | GenBank™accession number | Fragment length(bp) |
|---|---|---|---|
| GAPDH | ACCCAGAAGACTGTGGATGG | NM_001289726 | 186 |
| CACATTGGGGGTAGGAACAC | |||
| HSP27 | CCCAGTGAATCCCCTGTCTA | NM_001164708.1 | 179 |
| CCCCCAGGTTTTGGTTTATT | |||
| HSP70 | TGCTGATCCAGGTGTACGAG | NM_010478.2 | 204 |
| CGTTGGTGATGGTGATCTTG | |||
| HSP90 | GGCATCGATGAAGATGAGGT | NM_008302.3 | 196 |
| ACATGAGCAGAGAGCCAGGT | |||
| Smad1 | TCTTTCTGAAGTGGGCTTTC | NM_008539.3 | 185 |
| CAGCCAGCATACAGTTTCAG | |||
| Smad5 | TGTGCTCCTTGTGCAGATAA | NM_008541.3 | 221 |
| TGGTGCTCTACGAGACCTTC | |||
| OSX | CCCACCTAACAGGAGGATTT | NM_130458.3 | 200 |
| CACTGGAATGGAGTGAAACC | |||
| BMP2 | AGCAAGGACGTCGTGGTGCC | NM_007553.3 | 202 |
| ATTATTTCGGTGCTGGAAACTACT |
HSP, heat shock protein.