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. 2019 Dec 11;10:2766. doi: 10.3389/fimmu.2019.02766

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Copyright © 2019 Wickström, Lövgren, Volkmar, Reinhold, Duke-Cohan, Hartmann, Rebmann, Mueller, Melief, Maas, Ligtenberg, Hansson, Offringa, Seliger, Poschke, Reinherz and Kiessling.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of mutated peptides presented on the tumor cell surface. LC-DIAMS Poisson detection plots for neoepitopes from mutated (A) and wild-type (B) ENC1 and mutated (C) and wild-type (D) AGPS from 500,000 cells. Top black traces are extracted ion chromatograms for m/z of the doubly charged precursor ion in units of counts per second. The inverted traces (blue) are Poisson chromatograms showing the number of events, scaled 10-fold (as a convenience in plotting), that can be embedded at fixed cutoff probability in the MS/MS spectrum of the DIA window containing the precursor m/z (17). Nanospray MS3 Poisson detection of cysteine-containing neoantigen peptide CCT4 (E) or the corresponding wild-type peptide (F) was performed from 1.5 million cells, as marked with a 0-offset Poisson peak (18).