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. 2019 Dec 11;10:2766. doi: 10.3389/fimmu.2019.02766

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Copyright © 2019 Wickström, Lövgren, Volkmar, Reinhold, Duke-Cohan, Hartmann, Rebmann, Mueller, Melief, Maas, Ligtenberg, Hansson, Offringa, Seliger, Poschke, Reinherz and Kiessling.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neoepitopes detected by reverse immunology vs. mass spectrometry. With reverse immunology TIL reactivity against 5/181 (KADA) and 3/49 (ANRU) of the predicted neoepitopes were observed. With mass spectrometry 3/136 (KADA, not all peptides could be analyzed) and 1*/38 (ANRU, not all peptides could be analyzed) of the predicted neoepitopes were detected on the cell surface. KADA epitopes are in Bold, ANRU epitopes are in Italic. *Detected after IFNγ treatment of the tumor.