Fig. 5.
Viability and phenotypic effects after lincRNA knockout. (A) Offspring recovered at weaning from Rubie+/−×Rubie+/− cross versus expected Mendelian ratios. (B) Representative sporadic circling behavior in Rubie−/− offspring; #, only observed in Rubie−/− genotype over 2+ years of observation (left-handedness not exclusive in all observed cases). (C) Offspring recovered at weaning from Handlr+/−×Handlr+/− cross versus expected Mendelian ratios. (D) Offspring recovered at weaning from Atcayos+/−×Atcayos+/− cross versus expected Mendelian ratios; P-values in A, C and D derived from χ2 test. (E) LV mass calculated by echocardiographic measurements in Handlr wild-type versus null and in Atcayos wild-type versus null litter-matched adult males. (F) LV contractility measured by echocardiography in Handlr wild-type versus null and in Atcayos wild-type versus null litter-matched adult males. (G) Time course of cardiac contractility after TAC in Handlr wild-type versus null litter-matched adult males. (H) Time course of cardiac contractility after TAC in Atcayos wild-type versus null litter-matched adult males. (I) Week 8 post-TAC LV and lung weights in Handlr wild-type versus null litter-matched adult males. (J) Week 8 post-TAC LV and lung weights in Atcayos wild-type versus null litter-matched adult males. *P<0.05; **P<0.01; ***P<0.005; Student's two-tailed t-test, except I, Z-test. LV, left ventricle; FAC, fractional area shortening; TAC, transverse aortic constriction. Phenotypic data are mean±s.e.m. n=4-9 for all measurements.