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. 2019 Dec 5;8(12):bio047225. doi: 10.1242/bio.047225

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Activation of canonical WNT signaling in reprogrammed cells and pluripotent stem cells. (A) qRT-PCR results of the expression of WNT/β-CATENIN pathway components and target genes in MSCs and reprogrammed cells on day 14 under different conditions relative to H9-ESC expression. Bars represent the mean±s.d., n=3. Conditions with different letters are significantly different. (B) Heatmap showing the expression of canonical WNT/β-CATENIN pathway regulatory genes in MSCs, two iPSC lines (M₂O and LB₂O) and the day-5 EBs differentiated from these iPSCs. (C) qRT-PCR results of the expression levels of WNT/β-CATENIN target genes in MSCs, two iPSC lines (M₂O and LB₂O), the day-5 EBs differentiated from these iPSCs and H9-ESCs.