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. 2019 Dec 2;132(23):jcs237271. doi: 10.1242/jcs.237271

Fig. 1.

Fig. 1.

Atherosclerotic plaque macrophages contact agLDL that is highly enriched in FC. (A–D) Aortic sections from hyperlipidemic Apoe−/− mice containing atherosclerotic plaque were stained for FC using filipin (red) (A), for LDL using anti-ApoB antibody (green) and for macrophages using anti-F4/80 antibody (blue) (D). An overlay of filipin and ApoB staining (C) is also shown. (E–G) Aortic sections from hyperlipidemic Apoe−/− mice containing atherosclerotic plaque (E) were stained for free cholesterol using filipin (green) and for macrophages using anti-F4/80 antibody (red). Arrows indicate the location of the macrophage shown in F and G. (F) A single slice from a confocal z-stack showing a macrophage forming a LS around free cholesterol-enriched agLDL and showing orthogonal xz and yz planes. (G) 3D reconstruction of the macrophage shown in F. The agLDL (green and tan) is contained in a surface connected compartment.