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. 2019 Dec 4;146(23):dev176297. doi: 10.1242/dev.176297

Fig. 1.

Fig. 1.

Analysis of the cellular features of the mouse neuroepithelium. (A) Example clones in E11.5 embryos, data from Kicheva et al. (2014). Clonal labelling was induced at E9.5 of development. The coordinates of EYFP-labelled cells in the confocal image on the left are shown on the graph on the right. The AP/DV ratio of clones in the pMN domain (red marks) is higher than in the pD domain (green shades). Scale bar: 50 μm. (B) Top panels show the apical surface of E11.5 flat mounted mouse neural tube immunostained for ZO-1. Images were taken within the ventral (right) and dorsal (left) halves of the neural tube. Dorsal side up. Scale bar: 10 μm. Bottom panels show the segmented images after manual correction. Insets show an overlay of original and segmented image. (C) Histograms of apical area, perimeter, number of neighbours and elongation of cells from the dorsal (brown) and ventral (green) regions of E10.5 and E11.5 neural tubes. Sample sizes: E10.5, n=25 images of dorsal and five images of ventral domains from seven different embryos; E11.5, 11 images of dorsal, three images of ventral domains from three embryos.